Technical Downloads
Reference Standard Technical Manual & Laboratory Handling Guidebook
English | PDF | 8.9 MB
Manual Teknis Bahan Acuan & Panduan Penanganan Laboratorium
Indonesian | PDF | 9.7 MB
Notice & Intended Use: All technical documentation, protocols, and manuals provided by MarkHerb are intended strictly for scientific research and laboratory use. Products are not intended for human therapeutic, diagnostic, or clinical applications.
Technical Downloads:
Document Title | Format | File Size | Language | Quick Link |
|---|---|---|---|---|
Reference Standard Technical Manual & LaboratoryHandling Guidebook | 8.5 MB | English | ||
Manual Teknis Bahan Acuan & Panduan Penanganan Laboratorium | 8.9 MB | Indonesian |
Updated for 2026 • Optimized for Standard A4 & Letter Printing • ISO-Aligned SOPs
Notice & Intended Use: All technical documentation, protocols, and manuals provided by MarkHerb are intended strictly for scientific research and laboratory use. Products are not intended for human therapeutic, diagnostic, or clinical applications.
What's Inside The Manual?
(Content Overview)
Unboxing & Consolidating Micro-Quantities
This section outlines standard operating procedures for receiving and unboxing powder and liquid compounds. It provides step-by-step guidance on gentle tapping and micro-centrifugation (200–500 RPM) to ensure low-volume liquid droplets and fine powders consolidate fully at the base of the vial prior to seal break, minimizing product loss.
Storage & Stability Benchmark Matrix
Learn how to maintain compound integrity and prevent degradation over time. This section establishes thermal storage thresholds across 2–8 °C, -20 °C, and -80 °C for both dry powders and liquid stock solutions, while emphasizing single-use aliquoting strategies to eliminate harmful freeze-thaw cycles.
Analytical Solution Preparation SOP
Designed for high-precision analytical workflows, this module details micro-weighing protocols down to (0.01 mg) alongside strict tare drying guidelines (115 °C). It also provides comprehensive solubilization routines for poorly soluble compounds using organic solvents such as MeOH, MeCN, DCM, CHCl3, and THF.
Cell-Based & Enzyme Bioassay Workflows
Ensure cell viability and accurate bioassay results with safe co-solvent handling procedures. This section details stepwise dilution techniques that prevent localized precipitation while keeping final DMSO concentrations below < 0.5% v/v. It includes standardized molarity formulas to simplify working solution calculations:
In Vivo Formulation & Interspecies Dose Conversion
Translate experimental dosages accurately across animal species using FDA-aligned Body Surface Area (BSA) normalization factors (Km). This guide presents three distinct vehicle formulation schemes—co-solvent mixtures, SBE-β-CD inclusion complexes, and lipophilic oils, alongside the interspecies dose translation formula:
Thin-Layer Chromatography (TLC) & Solvent Miscibility
Verify compound identity and optimize mobile phase design with practical TLC instructions. This section covers plate preparation with a 4.0 cm elution path, UV 254 nm / 366 nm visualization protocols, Rf retention calculations, and a comprehensive solvent miscibility guide to prevent phase separation during analytical testing.
What's Inside The Manual?
(Content Overview)
Unboxing & Consolidating Micro-Quantities
This section outlines standard operating procedures for receiving and unboxing powder and liquid compounds. It provides step-by-step guidance on gentle tapping and micro-centrifugation (200–500 RPM) to ensure low-volume liquid droplets and fine powders consolidate fully at the base of the vial prior to seal break, minimizing product loss.
Storage & Stability Benchmark Matrix
Learn how to maintain compound integrity and prevent degradation over time. This section establishes thermal storage thresholds across 2–8 °C, -20 °C, and -80 °C for both dry powders and liquid stock solutions, while emphasizing single-use aliquoting strategies to eliminate harmful freeze-thaw cycles.
Analytical Solution Preparation SOP
Designed for high-precision analytical workflows, this module details micro-weighing protocols down to (0.01 mg) alongside strict tare drying guidelines (115 °C). It also provides comprehensive solubilization routines for poorly soluble compounds using organic solvents such as MeOH, MeCN, DCM, CHCl3, and THF.
Cell-Based & Enzyme Bioassay Workflows
Ensure cell viability and accurate bioassay results with safe co-solvent handling procedures. This section details stepwise dilution techniques that prevent localized precipitation while keeping final DMSO concentrations below < 0.5% v/v. It includes standardized molarity formulas to simplify working solution calculations:
In Vivo Formulation & Interspecies Dose Conversion
Translate experimental dosages accurately across animal species using FDA-aligned Body Surface Area (BSA) normalization factors (Km). This guide presents three distinct vehicle formulation schemes—co-solvent mixtures, SBE-β-CD inclusion complexes, and lipophilic oils, alongside the interspecies dose translation formula:
Thin-Layer Chromatography (TLC) & Solvent Miscibility
Verify compound identity and optimize mobile phase design with practical TLC instructions. This section covers plate preparation with a 4.0 cm elution path, UV 254 nm / 366 nm visualization protocols, Rf retention calculations, and a comprehensive solvent miscibility guide to prevent phase separation during analytical testing.
